How to Choose COOH, NH2 and SA Fluorescent Nanobeads
Surface groups define how fluorescent nanobeads connect with antibodies, antigens, biotinylated molecules and other targets. COOH, NH2 and SA systems should be matched to the coupling route and assay goal.
COOH beads
COOH beads are commonly used with EDC/NHS coupling and are a frequent starting point for antibodies, antigens and other protein targets.
NH2 beads
NH2 beads can be used in specific crosslinking systems depending on target molecule structure and stability requirements.
SA beads
SA beads are suitable for biotinylated antibodies, antigens or probes and provide flexible assay design options.
FAQ
Which group is suitable for initial development?
COOH is a common starting point for protein coupling. SA is convenient if biotinylated molecules are already available.
What information should be provided?
Target molecule, buffer system, reader, strip structure, sensitivity target and stability requirements are helpful.
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